Molecular Biology II – Omic Techniques

General

• Code: TABO-97-Θ7
• Semester: 9th
• Study Level: Undergraduate
• Course type: Skills Development
• Teaching and exams language: Greek (English for Erasmus students)
• The course is offered to Erasmus students
• Teaching Methods (Hours/Week): Lectures (3)
• ECTS Units: 4
• Class Schedule:
  • Wed 15:00 - 18:00:

Course Contents

• Introduction to the basic tools of Recombinant Technology DNA (Restriction endonucleases and modifying enzymes (classes, nomenclature, recognition sites).
• Cloning principles and systems (plasmids, bacteriophages, cosmids, BACs, YACs) 3) Nucleic acid isolation and analysis methods (DNA and RNA isolation methods, agarose/polyacrylamide gel, Southern, Northern blotting methods and hybridization principles).
• Genomic and cDNA libraries (construction, control and analysis of clones).
• Large-scale genomic approaches (DNA sequencing methods, random access sequencing, paired-end sequencing, next-generation sequencing – NGS. Microarrays).
• Polymerase chain reaction (PCR) ( Basic principles, primers, PCR- real-time, applications).
• Transcriptomics and metabolomics.
• Mutagenesis and gene inactivation.
• Prokaryotic-eukaryotic gene expression systems.
• Induction of gene expression (promoters and selection markers).
• Transfer of DNA into animal cells (chemical, mechanical, physical and biological methods. Ioi and bacilli).
• Genetic modification of animals (methods of producing transgenic mice, mammals and birds). Genome Transfer (Xenopus. Genome Transfer in Invertebrates: Transgenic Flies. Gene Therapy).
• RNA Interference (RNAi) and applications of the technique. Bioinsecticides.
• Comparison and analysis of genomes (Public databases of DNA, RNA and protein sequences. Comparative genomics).

Educational Goals

Upon successful completion of the course, the student will:

• He has understood the contribution of recombinant DNA technology to the rapid, and still continuing, progress of the life sciences.
• Familiar with technological molecular tools to study almost any organism.
• He will have used his theoretical knowledge to understand the working principles of molecular tools.
• Has understood the ways of creating mutations and their effects on the organism.
• Has developed his critical and creative thinking about the use of new technologies.
• Will be able to apply his knowledge to solve biological problems that will contribute to social progress.

General Skills

• Ability to design simple experiments.
• Independent and / or group work.
• Promotion of free, creative and inductive thinking.

Teaching Methods

• In the classroom, face to face.

Use of ICT means

• Basic software (windows, word, power point, the web, etc.).
• Support of learning process through the electronic platform / e-class.

Teaching Organization

Students Evaluation

• Written final exam (50%).
• Essay  (50%).

Recommended Bibliography

1. ΑνασυνδυασμένοDNA (2010) Συγγραφέας: Watson D.A. κα Εκδοτικός Οίκος: Ακαδημαϊκές εκδόσεις Ι Μπάσδρα & ΣΙΑ ΟΕ ISBN: 978-960-88412-5-3.