Courses

Analytical Biotechnology

General

Course Contents

Theoretical Part

  • Safety management of an analytical laboratory: Analytical biotechnology laboratory safety rules, waste disposal and management, good laboratory practice rules.
  • Evaluation and processing of analytical data: Introduction to sampling and chemometrics for the optimization of analytical measurements, reliability of analytical methods, reliability control and quality assurance program of measurements, Errors and statistical processing of analytical data, presentation of results, selection criteria and conditions for analytical method development and laboratory protocol.
  • Sample preparation: Basic principles of extraction – separation of biomolecules (liquid extraction methods, solid phase extraction, cold and hot extraction, with supercritical fluids, with ultrasound – microwaves, enzymes, dialysis, ultrafiltration, lyophilization).
  • Analytical methods and techniques: Centrifugal analytical techniques, Spectroscopy (ultraviolet-visible absorption spectrophotometry, infrared spectrophotometry, atomic spectrophotometry, molecular emission spectroscopy, molecular luminescence spectrometry, nuclear magnetic resonance spectroscopy, mass spectrometry, circular dichroism, polarimetry, tolerometry, nebulometry), chromatography (adsorption, distribution, gas chromatography, liquid chromatography), combined techniques (LC/MS, GC/MS, etc.), Electrophoretic analysis methods (isoelectric focusing, two-dimensional electrophoresis, capillary electrophoresis, immunoblotting), Electroanalytical analysis methods, Radioisotope analysis methods, Thermal analysis methods (differential scanning calorimetry, differential thermal analysis, thermogravimetric analysis, isothermal titration calorimetry), Enzymatic analysis methods, Nanosystems for ultrafast analysis, Proteomic analysis.

Laboratory Part

The laboratory part of the course consists of applications and examples of methods of analysis with agricultural and industrial interest.

Educational Goals

Upon successful completion of the course, the student will be able to:

  • Handles issues of laboratory safety, and disposal and management of laboratory waste.
  • Knows the principles of chemometry and sampling and evaluates and distinguishes errors, reliability and quality of laboratory measurements and applies the rules of good laboratory practice and the rules of reliability control and quality assurance of results.
  • Understands the basic principles of extraction and separation of biomolecules from various biological samples and the techniques used in the analysis of biomolecules and their importance in the identification, qualitative and quantitative analysis of biomolecules.
  • Choose the appropriate method for the study of unknown samples of biological interest and combine information from the various techniques for the qualitative and quantitative analysis of complex biological samples.
  • Develops the appropriate method for the study of unknown samples of biological interest and begins research on objects that require the use of the above laboratory techniques.
  • Collaborates in a team to prepare a study or protocol for the qualitative and quantitative analysis of an unknown biological sample, using interdisciplinary bibliographic data.

General Skills

  • Work autonomously.
  • Work in teams.
  •  Design and management of laboratory procedures.
  • Advance free, creative and causative thinking.

Teaching Methods

  • In the classroom, face to face.

Use of ICT means

  • Basic software (windows, word, power point, the web, etc.).
  • Support of learning process through the electronic platform / e-class.

Teaching Organization

ActivitySemester workload
Lectures26
Laboratory26
Laboratory reports/assignments73
Total125

Students Evaluation

  1. Written final exam (50%) including:
    • Comparative evaluation of theoretical background.
    • Development of the way of thinking.
  2. Written final laboratory exam (50%) which includes:
    • Comparative evaluation of elements of laboratory exercises.
    • Development of the way of thinking.

Recommended Bibliography

  1. Βιβλίο [86053293]: Βιοτεχνολογία-Βασικές Αρχές και Εφαρμογές, Renneberg Reinhard, Berkling Viola, Loroch Vanya, Süßbier Darja.
  2. Βιβλίο [41965278]: ΦΑΡΜΑΚΕΥΤΙΚΗ ΑΝΑΛΥΣΗ: ΕΝΑ ΣΥΓΓΡΑΜΜΑ ΓΙΑ ΦΟΙΤΗΤΕΣ, D.G. WATSON.
  3. Schalkhammer, Thomas (2002) Analytical Biotechnology, ISBN 978-3-0348-8101-2.
  4. Holme D. and Peck H. (2001) Analytical Biochemistry, Pearson Education Editions.
  5. Keith Wilson and John Walker (2000) Principles and Techniques of Practical Biochemistry (5th edition), Cambridge University Press.
  6. P.R. Yadav (2005) Experimental Biotechnology, Discovery Publishing House.
  7. Rodney F. Boyer (2000), Modern Experimental Biochemistry (3rd Edition) Addison Wesley Longman.

Related Research Journals

  1. Analytical Biotechnology.
  2. Analytical and Bioanalytical Chemistry.